| 1 | Olerex AS | 392 557 690 |
| 2 | Cronimet Nordic OÜ | 143 997 911 |
| 3 | Eesti Viljasalv OÜ | 117 121 045 |
| 4 | Apteekide Koostöö Hulgimüük OÜ | 92 114 353 |
| 5 | Tartu Tarbijate Kooperatiiv TuÜ | 80 270 789 |
| 6 | Põllumeeste ühistu Kevili | 77 583 575 |
| 7 | MapriEhitus OÜ | 74 872 800 |
| 8 | A. Le Coq AS | 71 287 000 |
| 9 | Palmako AS | 57 778 028 |
| 10 | Ehitustrust AS | 57 019 294 |
| 11 | Decora AS | 56 360 392 |
| 12 | Hanza Mechanics Tartu AS (Edukas Eesti Ettevõte 2020) | 53 571 000 |
| 13 | Ehitusfirma Rand ja Tuulberg AS | 51 485 791 |
| 14 | Kodumajatehase AS | 49 563 790 |
| 15 | Tartu Mill AS | 45 081 330 |
| 16 | Lasita Maja OÜ | 37 868 954 |
| 17 | Fortumo OÜ | 35 153 325 |
| 18 | Playtech Estonia OÜ | 34 977 682 |
| 19 | A&P Mets AS | 31 837 774 |
| 20 | Estiko-Plastar AS | 31 331 640 |
| 21 | Maag Grupp AS | 31 198 000 |
| 22 | Kodumaja AS | 31 005 459 |
| 23 | KM Element OÜ | 28 883 901 |
| 24 | Kroonpress AS | 28 182 424 |
| 25 | Aasta Auto AS | 28 087 463 |
| 26 | Henkel Balti OÜ | 25 646 729 |
| 27 | Eviko AS | 25 499 190 |
| 28 | Tartu Keskkatlamaja AS | 24 076 675 |
| 29 | Gobus AS | 24 042 694 |
| 30 | EmbachEhitus OÜ | 22 969 041 |
| 31 | GRK Infra AS | 22 806 088 |
| 32 | Karl Bilder OÜ | 22 591 908 |
| 33 | Tarmetec OÜ (Edukas Eesti Ettevõte 2019) | 21 786 964 |
| 34 | TMB Element OÜ | 20 892 179 |
| 35 | Lemeks Wood Supply AS | 18 753 490 |
| 36 | Autospirit Tartu OÜ | 18 666 219 |
| 37 | Salvest AS | 18 567 187 |
| 38 | Tref AS | 16 510 046 |
| 39 | Tartu Ehitus AS | 16 044 411 |
| 40 | Tiksoja Puidugrupp AS | 15 843 652 |
| 41 | Recar Group OÜ | 15 765 875 |
| 42 | Nason Davis Eesti OÜ | 15 092 779 |
| 43 | Aqua Marina AS | 14 688 715 |
| 44 | Truck Trading Estonia OÜ | 14 602 951 |
| 45 | Forestex Tartu OÜ | 14 381 404 |
| 46 | Metaan AS | 14 288 832 |
| 47 | Balt-Hellin AS | 13 646 416 |
| 48 | Tartu Uusapteek OÜ | 13 625 017 |
| 49 | Data Print OÜ | 13 290 949 |
| 50 | Aasta Auto Pluss AS | 13 283 854 |
